Lysinibacillus macroides sp. nov., nom. rev

Int J Syst Evol Microbiol. 2012 May;62(Pt 5):1121-1127. doi: 10.1099/ijs.0.027995-0. Epub 2011 Jul 1.

Abstract

'Bacillus macroides' ATCC 12905(T) ( = DSM 54(T) = LMG 18474(T)), isolated in 1947 from cow dung, was not included in the Approved Lists of Bacterial Names and so it lost standing in bacteriological nomenclature. Reinvestigation of the strain, including DNA-DNA relatedness experiments, revealed that 'Bacillus macroides' is genomically distinct from its closest relatives Lysinibacillus xylanilyticus, Lysinibacillus boronitolerans and Lysinibacillus fusiformis (as determined by 16S rRNA gene sequence analysis, with pairwise similarity values of 99.2, 98.8 and 98.5 %, respectively, with the type strains of these species). Further analysis showed that 'Bacillus macroides' shares the A4α L-Lys-D-Asp peptidoglycan type with other members of the genus Lysinibacillus and can thus be attributed to this genus. These results, combined with additional phenotypic data, justify the description of strain LMG 18474(T) ( = DSM 54(T) = ATCC 12905(T)) as Lysinibacillus macroides sp. nov., nom. rev.

MeSH terms

  • Animals
  • Bacillaceae / chemistry
  • Bacillaceae / classification*
  • Bacillaceae / genetics
  • Bacillaceae / isolation & purification*
  • Bacterial Typing Techniques
  • Cattle
  • Cluster Analysis
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Feces / microbiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Peptidoglycan / chemistry
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Peptidoglycan
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/AJ628749