The ubiquitous plasmid pXap41 in the invasive phytopathogen Xanthomonas arboricola pv. pruni: complete sequence and comparative genomic analysis

FEMS Microbiol Lett. 2011 Oct;323(1):52-60. doi: 10.1111/j.1574-6968.2011.02352.x. Epub 2011 Jul 27.

Abstract

The complete DNA sequence of the 41 102-bp plasmid pXap41 from the invasive plant pathogen Xanthomonas arboricola pv. pruni CFBP 5530 was determined and its 44 coding regions were annotated. Comparative analysis with 15 Xanthomonas plasmids and 19 complete genomes revealed that nearly one-fourth of this plasmid has high sequence identity to plasmid pXAC64 and an 8.8-kb chromosomal region of Xanthomonas axonopodis pv. citri strain 306 carrying genes that encode type III effectors and helper proteins. The presence of pXap41 in all X. arboricola pv. pruni genotypes was confirmed for eight strains by plasmid profiling and for 35 X. arboricola pv. pruni isolates with a new plasmid multiplex PCR assay. This plasmid was not detected in any other X. arboricola pathovars (n=12), indicating the potential for the application of the pXap41 PCR method as a pathovar-level detection and identification tool.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Bacterial / chemistry*
  • DNA, Bacterial / genetics*
  • Evolution, Molecular
  • Gene Order
  • Molecular Sequence Data
  • Open Reading Frames
  • Plasmids*
  • Sequence Analysis, DNA
  • Sequence Homology
  • Xanthomonas axonopodis / genetics*

Substances

  • DNA, Bacterial

Associated data

  • GENBANK/FR875157