Abstract
An epidemiological survey on a Theileria parasite infection of cattle in Northeast China was carried out using allele-specific PCR and DNA sequence analysis of the major piroplasm surface protein (MPSP) gene. The results showed that 14 of 104 blood samples were positive for Theileria by PCR. Among the positive cases, co-infection with various combinations of C- and I-type parasites was detected in 12 samples; no B- and Thai-type parasites were detected by allele-specific PCR. Phylogenetic analysis based on the MPSP gene sequences revealed that Theileria parasites with the MPSP types 1, 2, and 4 were distributed in Northeast China.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antigens, Protozoan / genetics
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Base Sequence
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Cattle
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China / epidemiology
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DNA, Protozoan / analysis
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Molecular Epidemiology
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Molecular Sequence Data
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Phylogeny
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Polymerase Chain Reaction / veterinary
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Protozoan Proteins / genetics
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Sequence Analysis, DNA / veterinary
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Theileria / genetics*
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Theileria / isolation & purification
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Theileriasis / epidemiology*
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Theileriasis / parasitology
Substances
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Antigens, Protozoan
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DNA, Protozoan
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Protozoan Proteins
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major piroplasm surface protein, Theileria
Associated data
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GENBANK/HQ322618
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GENBANK/HQ322619
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GENBANK/HQ322620
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GENBANK/HQ322621