Abstract
In silico analysis of nucleotide sequences flanking the recently found hydroquinone dioxygenase in Sphingomonas sp. strain TTNP3 revealed a gene cluster that encodes a hydroquinone catabolic pathway. In addition to the two open-reading frames encoding the recently characterized hydroquinone dioxygenase, the cluster consisted of six open-reading frames. We were able to express the three open-reading frames, hqdC, hqdD, and hqdE, and demonstrated that the three gene products, HqdC, HqdD, and HqdE had 4-hydroxymuconic semialdehyde dehydrogenase, maleylacetate reductase, and intradiol dioxygenase activity, respectively. Surprisingly, the gene cluster showed similarities to functionally related clusters found in members of the β- and γ-proteobacteria rather than to those found in other members of the genus Sphingomonas sensu latu.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Biodegradation, Environmental
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Biotechnology
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Dioxygenases / genetics
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Dioxygenases / metabolism
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Fatty Acids, Unsaturated / metabolism
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Genes, Bacterial
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Hydroquinones / metabolism*
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Molecular Sequence Data
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Multigene Family / genetics*
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Oxidoreductases / genetics
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Oxidoreductases / metabolism
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Oxidoreductases Acting on CH-CH Group Donors / genetics
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Oxidoreductases Acting on CH-CH Group Donors / metabolism
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Phenols / metabolism
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Sequence Analysis, DNA
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Sphingomonas / enzymology*
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Sphingomonas / genetics
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Sphingomonas / growth & development
Substances
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Bacterial Proteins
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Fatty Acids, Unsaturated
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Hydroquinones
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Phenols
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hydroxymuconic semialdehyde
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Oxidoreductases
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Dioxygenases
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Oxidoreductases Acting on CH-CH Group Donors
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maleylacetate reductase
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hydroquinone