Abstract
We report multiplex ligation-dependent probe amplification analysis (MLPA) of DNA copy number alterations in 59 esophageal cancer samples, stratified by histotype. Results showed that squamous cell carcinoma (SCC) samples present clustered abnormalities with several genes altered at high frequency. Instead, esophageal adenocarcinoma (ADC) samples are characterized by a more widespread genomic instability, and in these patients total DNA copy number alterations resulted to be an independent prognostic factor. The detection of characteristic molecular changes represents a step towards a better understanding of the molecular basis of esophageal tumorigenesis, and might offer the potential for the discovery of tumor-specific biomarkers.
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenocarcinoma / diagnosis
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Adenocarcinoma / genetics*
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Adult
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Aged
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Aged, 80 and over
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Carcinoma, Squamous Cell / diagnosis
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Carcinoma, Squamous Cell / genetics*
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Chromosome Aberrations
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Cyclin-Dependent Kinase Inhibitor p15 / genetics
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Cyclin-Dependent Kinase Inhibitor p16 / genetics
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DNA, Neoplasm / genetics*
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Diagnosis, Differential
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Esophageal Neoplasms / diagnosis
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Esophageal Neoplasms / genetics*
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Female
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Gene Amplification
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Gene Deletion
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Gene Dosage
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Humans
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Kaplan-Meier Estimate
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Male
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Middle Aged
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Nucleic Acid Amplification Techniques / methods
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Oligonucleotide Probes / genetics
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Prognosis
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Vascular Endothelial Growth Factor A / genetics
Substances
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CDKN2B protein, human
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Cyclin-Dependent Kinase Inhibitor p15
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Cyclin-Dependent Kinase Inhibitor p16
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DNA, Neoplasm
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Oligonucleotide Probes
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Vascular Endothelial Growth Factor A