Interrogating the repertoire: broadening the scope of peptide-MHC multimer analysis

Nat Rev Immunol. 2011 Jul 15;11(8):551-8. doi: 10.1038/nri3020.

Abstract

Labelling antigen-specific T cells with peptide-MHC multimers has provided an invaluable way to monitor T cell-mediated immune responses. A number of recent developments in this technology have made these multimers much easier to make and use in large numbers. Furthermore, enrichment techniques have provided a greatly increased sensitivity that allows the analysis of the naive T cell repertoire directly. Thus, we can expect a flood of new information to emerge in the coming years.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Cancer Vaccines / immunology
  • Cell Tracking / methods
  • Communicable Diseases / immunology
  • Epitope Mapping / methods
  • Epitope Mapping / trends
  • Epitopes, T-Lymphocyte / analysis*
  • Epitopes, T-Lymphocyte / immunology
  • Flow Cytometry / methods
  • Humans
  • Immunologic Techniques / trends
  • Immunotherapy, Adoptive / methods
  • Immunotoxins / immunology
  • Immunotoxins / therapeutic use
  • Major Histocompatibility Complex / immunology*
  • Mass Spectrometry / methods
  • Mice
  • Peptides / immunology*
  • Receptors, Antigen, T-Cell / immunology
  • T-Lymphocyte Subsets / cytology
  • T-Lymphocyte Subsets / immunology
  • T-Lymphocytes / cytology
  • T-Lymphocytes / immunology*
  • Vaccines / immunology

Substances

  • Cancer Vaccines
  • Epitopes, T-Lymphocyte
  • Immunotoxins
  • Peptides
  • Receptors, Antigen, T-Cell
  • Vaccines