Identification of Aeromonas isolates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

Brigitte Lamy; Angeli Kodjo; Frédéric Laurent; ColBVH Study Group

doi:10.1016/j.diagmicrobio.2011.04.014

Identification of Aeromonas isolates by matrix-assisted laser desorption ionization time-of-flight mass spectrometry

Diagn Microbiol Infect Dis. 2011 Sep;71(1):1-5. doi: 10.1016/j.diagmicrobio.2011.04.014. Epub 2011 Jul 16.

Authors

Brigitte Lamy¹, Angeli Kodjo, Frédéric Laurent; ColBVH Study Group

Collaborators

ColBVH Study Group:
F Carmagnol, E Chachaty, C Alba-Sauviat, C Auvray, D Barraud, Z Benseddik, A Bertrou, F Bessis, H Biessy, V Blanc, Y Boucaud-Maitre, P Brunet, A Michel, B Cancet, J Carrere, A Cecille, G Chambreuil, P Chantelat, H Chardon, C Charrel, H De Montclos, J W Decousser, J M Delarbre, A Gravet, D Deligne, C Denoix, J Deregnaucourt, F Desroys du Roure, S Dubourdieu, Z El Harrif, C Eloy, A Evers, C Febvre, D Fevre, S Gabriel, M J Galanti, M Gavignet, F Geffroy, G Grise, I Gros, I Hermes, J Heurte, E Heusse, D Jan, E Jaouen, S Laluque, R Lamarca, B Lamy, Laurens, A Le Coustumier, E Lecaillon, C Lemble, M Leneveu, S Leotard, M N Letouzey, C Malbrunot, O Menouni, M Morel, C Olive, B Pangon, J G Paul, J M Perez, P Pouedras, D Pressac, R Sanchez, Y Scat, A Secher, J Semon, D Simeon, C Simonin, J P Thellier, B Tourand, A Vachée, C Varache, J Vaucel, A C Vautrin, A Verhaeghe, M Villemain, L Villeneuve

Affiliation

¹ Centre Hospitalier Universitaire de Montpellier, Laboratoire de Bactériologie, Hôpital Arnaud de Villeneuve, Montpellier, France. [email protected]

PMID: 21763094
DOI: 10.1016/j.diagmicrobio.2011.04.014

Abstract

We evaluated the accuracy of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for identifying aeromonads with an extraction procedure. Genus-level accuracy was 100%. Compared to rpoB gene sequencing, species-level accuracy was 90.6% (29/32) for type and reference strains and 91.4% for a collection of 139 clinical and environmental isolates, making this system one of the most accurate and rapid methods for phenotypic identification. The reliability of this technique was very promising, although some improvements in database composition, taxonomy, and discriminatory power are needed.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aeromonas / classification
Aeromonas / isolation & purification*
Bacterial Typing Techniques / economics
Databases, Factual
Reproducibility of Results
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization* / economics