Structural mass spectrometry of proteins using hydroxyl radical based protein footprinting

Liwen Wang; Mark R Chance

doi:10.1021/ac200567u

Structural mass spectrometry of proteins using hydroxyl radical based protein footprinting

Anal Chem. 2011 Oct 1;83(19):7234-41. doi: 10.1021/ac200567u. Epub 2011 Aug 1.

Authors

Liwen Wang¹, Mark R Chance

Affiliation

¹ Center for Proteomics & Bioinformatics, School of Medicine, Case Western Reserve University, Cleveland, Ohio 44106, USA.

Abstract

Structural MS is a rapidly growing field with many applications in basic research and pharmaceutical drug development. In this feature article the overall technology is described and several examples of how hydroxyl radical based footprinting MS can be used to map interfaces, evaluate protein structure, and identify ligand dependent conformational changes in proteins are described.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Hydroxyl Radical / chemistry*
Mass Spectrometry
Models, Molecular
Protein Conformation
Protein Footprinting*
Proteins / chemistry*

Substances

Proteins
Hydroxyl Radical

Abstract

Publication types

MeSH terms

Substances

Grants and funding