Abstract
Cre-loxP recombination is widely used for genetic manipulation of the mouse genome. Here, we report generation and characterization of a new Cre line, Stella-Cre, where Cre expression cassette was targeted to the 3' UTR of the Stella locus. Stella is specifically expressed in preimplantation embryos and in the germline. Cre-loxP recombination efficiency in Stella-Cre mice was investigated at several genomic loci including Rosa26, Jak2, and Npm1. At all the loci examined, we observed 100% Cre-loxP recombination efficiency in the embryos and in the germline. Thus, Stella-Cre mice serve as a very efficient deleter line.
Copyright © 2011 Wiley-Liss, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions / genetics
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Animals
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Attachment Sites, Microbiological / genetics
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Bacteriophage P1 / genetics
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Blastocyst
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Chromosomal Proteins, Non-Histone
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Embryo, Mammalian / metabolism
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Female
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Flow Cytometry
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Gene Expression*
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Germ Cells / metabolism
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Integrases / genetics*
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Integrases / metabolism
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Janus Kinase 2 / genetics
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Male
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Mice
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Mice, 129 Strain
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Mice, Transgenic
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Nuclear Proteins / genetics
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Nucleophosmin
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Proteins / genetics
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RNA, Untranslated
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Recombination, Genetic*
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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beta-Galactosidase / genetics
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beta-Galactosidase / metabolism
Substances
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3' Untranslated Regions
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Chromosomal Proteins, Non-Histone
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Dppa3 protein, mouse
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Gt(ROSA)26Sor non-coding RNA, mouse
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Npm1 protein, mouse
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Nuclear Proteins
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Proteins
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RNA, Untranslated
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Repressor Proteins
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Nucleophosmin
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Jak2 protein, mouse
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Janus Kinase 2
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Cre recombinase
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Integrases
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beta-Galactosidase