Tanghinigenin from seeds of Cerbera manghas L. induces apoptosis in human promyelocytic leukemia HL-60 cells

Guo-Fei Wang; Yue-Wei Guo; Bo Feng; Liang Li; Cai-Guo Huang; Bing-Hua Jiao

doi:10.1016/j.etap.2010.03.012

Tanghinigenin from seeds of Cerbera manghas L. induces apoptosis in human promyelocytic leukemia HL-60 cells

Environ Toxicol Pharmacol. 2010 Jul;30(1):31-6. doi: 10.1016/j.etap.2010.03.012. Epub 2010 Mar 17.

Authors

Guo-Fei Wang¹, Yue-Wei Guo, Bo Feng, Liang Li, Cai-Guo Huang, Bing-Hua Jiao

Affiliation

¹ Department of Biochemistry and Molecular Biology, Second Military Medical University, 800 Xiangyin Road, Shanghai 200433, People's Republic of China.

PMID: 21787626
DOI: 10.1016/j.etap.2010.03.012

Abstract

Tanghinigenin, a cardiac glycoside, is isolated from the seeds of Cerbera manghas L. In this study, we demonstrated that tanghinigenin reduced the viability of human promyelocytic leukemia HL-60 cells in a time- and dose-dependent manner, and efficiently induced apoptosis in HL-60 cells as evidenced by the Annexin V/PI binding assay, DNA fragmentation and AO/EB staining studies. In addition, stimulation of HL-60 cells with tanghinigenin induced a series of intracellular events including the activation of caspase-3, -8, and -9, as well as up-regulation of Fas and FasL protein level. Taken together, caspase activation and Fas/FasL interaction was found to be involved in tanghinigenin-induced HL-60 cell apoptosis.