Surfactant protein D and KL-6 serum levels in systemic sclerosis: correlation with lung and systemic involvement

Sarcoidosis Vasc Diffuse Lung Dis. 2011 Jul;28(1):27-33.

Abstract

Objective: The aim of this study was to investigate the relationship between SP-D and KL-6 serum concentrations and the extent of interstitial lung involvement, as measured by a quantitative HRCT score and the functional impairment, in patients with systemic sclerosis (SSc). Moreover we analysed the association between these lung-specific biomarkers and skin involvement, anti-Scl-70 antibody titres and an index of disease activity.

Methods: Serum SP-D, KL-6 and anti-Scl-70 concentrations were determined by ELISA in 25 SSc patients. Disease activity and lung function parameters were assessed, and the extent of ILD was measured by a HRCT score.

Results: SP-D and KL-6 concentrations were higher in patients with SSc and lung fibrosis than in healthy controls. KL-6 correlated positively with the HRCT-fibrosis score (r=0.68, p<0.001), SP-D showed a weaker correlation (r=0.44, p=0.025). Increased KL-6 concentrations were associated with decreased DLCO and decreased FVC in SSc patients, SP-D showed no association. Furthermore KL-6, but not SP-D, showed a strong association with skin involvement as expressed by the modified Rodnan skin score (r=0.71, p<0.0001) and a disease activity index (r=0.73, p<0.0001).

Conclusion: KL-6 is more strongly associated than SP-D with the HRCT-fibrosis score, and, different from SP-D, it correlates with skin involvement and disease activity. We suggest that KL-6 may be a useful biomarker in the assessment of scleroderma patients.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoantibodies / blood*
  • Biomarkers / blood
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Follow-Up Studies
  • Humans
  • Lung / physiopathology*
  • Male
  • Middle Aged
  • Mucin-1 / blood*
  • Prognosis
  • Pulmonary Fibrosis / blood*
  • Pulmonary Fibrosis / complications
  • Pulmonary Fibrosis / physiopathology
  • Pulmonary Surfactant-Associated Protein D / blood*
  • Respiratory Function Tests
  • Scleroderma, Systemic / blood*
  • Scleroderma, Systemic / complications
  • Severity of Illness Index

Substances

  • Autoantibodies
  • Biomarkers
  • MUC1 protein, human
  • Mucin-1
  • Pulmonary Surfactant-Associated Protein D