Synthesis of purine-scaffold fluorescent probes for heat shock protein 90 with use in flow cytometry and fluorescence microscopy

Bioorg Med Chem Lett. 2011 Sep 15;21(18):5347-52. doi: 10.1016/j.bmcl.2011.07.026. Epub 2011 Jul 14.

Abstract

Fluorescent ligands for the heat shock protein 90 (Hsp90) were synthesized containing either fluorescein isothiocyanate (FITC), 4-nitrobenzo[1,2,5]oxadiazole (NBD) or the red shifted dye sulforhodamine 101 (Texas Red) conjugated to PU-H71. Two of the compounds, PU-H71-FITC2 (9) and PU-H71-NBD1 (8), were shown to be suitable for fluorescence-activated flow cytometry and fluorescence microscopy. Thus these molecules serve as useful probes for studying Hsp90 in heterogeneous live cell populations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Chemistry Techniques, Synthetic
  • Flow Cytometry
  • Fluorescent Dyes / chemical synthesis*
  • Fluorescent Dyes / chemistry*
  • HSP90 Heat-Shock Proteins / analysis*
  • Humans
  • Microscopy, Fluorescence
  • Molecular Structure
  • Purines / chemistry*
  • Stereoisomerism

Substances

  • Fluorescent Dyes
  • HSP90 Heat-Shock Proteins
  • Purines
  • purine