Synthesis and functionalization of persistent luminescence nanoparticles with small molecules and evaluation of their targeting ability

Thomas Maldiney; Gerardo Byk; Nicolas Wattier; Johanne Seguin; Raz Khandadash; Michel Bessodes; Cyrille Richard; Daniel Scherman

doi:10.1016/j.ijpharm.2011.06.048

Synthesis and functionalization of persistent luminescence nanoparticles with small molecules and evaluation of their targeting ability

Int J Pharm. 2012 Feb 14;423(1):102-7. doi: 10.1016/j.ijpharm.2011.06.048. Epub 2011 Jul 23.

Authors

Thomas Maldiney¹, Gerardo Byk, Nicolas Wattier, Johanne Seguin, Raz Khandadash, Michel Bessodes, Cyrille Richard, Daniel Scherman

Affiliation

¹ Unité de Pharmacologie Chimique et Génétique et d'Imagerie, CNRS, UMR 8151, F-75270 Paris Cedex, France.

PMID: 21803143
DOI: 10.1016/j.ijpharm.2011.06.048

Abstract

We have recently reported the design and use of inorganic nanoparticles with persistent luminescence properties. Such nanoparticles can be excited with a UV lamp for 2min and emit light in the near-infrared area for dozen of minutes without any further excitation. This property is of particular interest for small animal optical imaging, since it avoids the autofluorescence of endogenous fluorophores which is one major problem encountered when using fluorescent probes. We report herein the synthesis of persistent luminescence nanoparticles (PLNPs) and their functionalization with two small targeting molecules: biotin and Rak-2. We provide characterization of each PLNP as well as preliminary evidence of the ability of PLNP-PEG-Biotin to target streptavidin and PLNP-PEG-Rak-2 to bind prostate cancer cells in vitro.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biotin / chemistry
Calcium Chloride / chemistry
Cell Line, Tumor
Chemistry Techniques, Synthetic / methods*
Chlorides / administration & dosage
Chlorides / chemistry
Diagnostic Imaging / methods*
Drug Delivery Systems / methods*
Drug Stability
Dysprosium / administration & dosage
Dysprosium / chemistry
Europium / administration & dosage
Europium / chemistry
Humans
Luminescent Agents / administration & dosage*
Luminescent Agents / chemistry
Magnesium Compounds / chemistry
Male
Manganese Compounds / administration & dosage
Manganese Compounds / chemistry
Nanoparticles / chemistry*
Nitrates / administration & dosage
Nitrates / chemistry
Polyethylene Glycols / chemistry
Prostatic Neoplasms
Protein Binding
Silanes / chemistry
Spectroscopy, Fourier Transform Infrared
Static Electricity
Streptavidin / chemistry
Surface Properties
Thermogravimetry
Zinc Compounds / chemistry

Substances

Chlorides
Luminescent Agents
Magnesium Compounds
Manganese Compounds
Nitrates
Silanes
Zinc Compounds
dysprosium nitrate
Dysprosium
Polyethylene Glycols
tetraethoxysilane
Europium
Biotin
magnesium nitrate
zinc chloride
Streptavidin
Calcium Chloride
manganese chloride