Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

Sam Abraham; James Chin; Huub J M Brouwers; Bernadette Turner; Ren Zhang; Toni A Chapman

doi:10.1128/AEM.00534-11

Green fluorescent protein-based biosensor to detect and quantify stress responses induced by DNA-degrading colicins

Appl Environ Microbiol. 2011 Sep;77(18):6691-3. doi: 10.1128/AEM.00534-11. Epub 2011 Jul 29.

Authors

Sam Abraham¹, James Chin, Huub J M Brouwers, Bernadette Turner, Ren Zhang, Toni A Chapman

Affiliation

¹ Microbiological Diseases and Diagnostics Research, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, New South Wales, Australia.

Abstract

Here we report the development of a whole-cell biosensor to detect and quantify the induction of the SOS response activated by DNA-degrading colicins. This biosensor utilizes the SOS-responsive cda promoter to regulate the expression of green fluorescent protein. The biosensor assay revealed induction of stress for all DNA-degrading reference colicins (E2, E7, and E8).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biosensing Techniques / methods*
Colicins / toxicity*
DNA Damage / drug effects*
Escherichia coli / drug effects*
Escherichia coli / genetics
Escherichia coli / physiology
Gene Expression Regulation, Bacterial
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism*
Promoter Regions, Genetic
SOS Response, Genetics*
Transcription, Genetic

Substances

Colicins
Green Fluorescent Proteins