Abstract
Hypoxia-inducible factor-1 alpha (HIF-1α) is a master transcription factor that regulates the response to hypoxia and ischemia and induces the expression of various genes, including vascular endothelial growth factor (VEGF) and erythropoietin (EPO). This study shows the systemic response of increased HIF-1α, EPO, and VEGF mRNA and protein. In addition, VEGF expression was increased in neurons and over-expressed in glial cells in a model of neuroexcitotoxicity in the hippocampus, in which rats were neonatally exposed to high glutamate concentrations. Simultaneous increases in HIF-1α, EPO and VEGF mRNA in peritoneal macrophages were also observed. Our study is consistent with the hypothesis that these genes exert a protective effect in response to neurotoxicity.
Copyright © 2011 Elsevier B.V. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Age Factors
-
Animals
-
Animals, Newborn
-
Disease Models, Animal
-
Erythropoietin / genetics
-
Erythropoietin / metabolism
-
Female
-
Gene Expression Regulation, Developmental / drug effects
-
Gene Expression Regulation, Developmental / physiology*
-
Glial Fibrillary Acidic Protein / metabolism
-
Glutamic Acid / toxicity
-
Hippocampus / drug effects
-
Hippocampus / metabolism*
-
Hippocampus / pathology
-
Hypoxia-Inducible Factor 1, alpha Subunit / genetics
-
Hypoxia-Inducible Factor 1, alpha Subunit / metabolism*
-
Macrophages / drug effects
-
Macrophages / metabolism*
-
Male
-
Neurons / drug effects
-
Neurons / metabolism
-
Neurotoxicity Syndromes / etiology
-
Neurotoxicity Syndromes / pathology*
-
Neurotoxins / toxicity
-
Pregnancy
-
RNA, Messenger
-
Rats
-
Rats, Wistar
-
Vascular Endothelial Growth Factor A / genetics
-
Vascular Endothelial Growth Factor A / metabolism
Substances
-
Glial Fibrillary Acidic Protein
-
Hif1a protein, rat
-
Hypoxia-Inducible Factor 1, alpha Subunit
-
Neurotoxins
-
RNA, Messenger
-
Vascular Endothelial Growth Factor A
-
Erythropoietin
-
Glutamic Acid