Biosynthetic origin and mechanism of formation of the aminoribosyl moiety of peptidyl nucleoside antibiotics

J Am Chem Soc. 2011 Sep 14;133(36):14452-9. doi: 10.1021/ja206304k. Epub 2011 Aug 22.

Abstract

Several peptidyl nucleoside antibiotics that inhibit bacterial translocase I involved in peptidoglycan cell wall biosynthesis contain an aminoribosyl moiety, an unusual sugar appendage in natural products. We present here the delineation of the biosynthetic pathway for this moiety upon in vitro characterization of four enzymes (LipM-P) that are functionally assigned as (i) LipO, an L-methionine:uridine-5'-aldehyde aminotransferase; (ii) LipP, a 5'-amino-5'-deoxyuridine phosphorylase; (iii) LipM, a UTP:5-amino-5-deoxy-α-D-ribose-1-phosphate uridylyltransferase; and (iv) LipN, a 5-amino-5-deoxyribosyltransferase. The cumulative results reveal a unique ribosylation pathway that is highlighted by, among other features, uridine-5'-monophosphate as the source of the sugar, a phosphorylase strategy to generate a sugar-1-phosphate, and a primary amine-requiring nucleotidylyltransferase that generates the NDP-sugar donor.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / biosynthesis*
  • Anti-Bacterial Agents / chemistry
  • Anti-Bacterial Agents / pharmacology
  • Biological Products / chemical synthesis
  • Biological Products / chemistry
  • Glycosylation
  • Nucleosides / biosynthesis*
  • Nucleosides / chemistry
  • Nucleosides / pharmacology
  • Nucleotidyltransferases / chemistry*
  • Pentosyltransferases / chemistry*
  • Transaminases / chemistry*
  • Uridine Phosphorylase

Substances

  • Anti-Bacterial Agents
  • Biological Products
  • Nucleosides
  • Pentosyltransferases
  • Uridine Phosphorylase
  • deoxyuridine phosphorylase
  • Transaminases
  • Nucleotidyltransferases