Abstract
Fritillaria ussuriensis (FU, derived from the bulbs of various species of the genus Fritillaria, including Fritillaria thunbergii Miq.) is used in herbal medicine to treat conditions such as eczema, skin burns, and frostbite. In this study, we investigated the mechanism of the anti-allergy effect of FU. FU extract (80 mg/kg), orally administered to Sprague-Dawley (SD) rats, significantly inhibited the passive cutaneous anaphylaxis (PCA) reaction. It inhibited the compound 48/80-induced release of histamine from rat peritoneal mast cells in a concentration-dependent manner. Significant inhibitory effects of the FU extract on IL-6, IL-8, and TNF-α (1, 10, and 100 µg/mL) were observed in HMC-1 cells. Treatment with FU attenuated PMA plus A23187-induced phosphorylation of all three MAPKs, especially at concentrations of 10 and 100 µg/mL. Further, it (80 mg/kg) led to significant inhibition of mast-cell accumulation in ear tissue at the chronic phase. These results indicate that it inhibits allergic reactions.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Administration, Oral
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Animals
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Anti-Allergic Agents / chemistry
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Anti-Allergic Agents / pharmacology*
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Anti-Allergic Agents / therapeutic use
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Calcimycin / pharmacology
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Fritillaria / chemistry*
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Histamine Release / drug effects*
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Histamine Release / immunology
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Hypersensitivity / drug therapy*
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Hypersensitivity / immunology
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Hypersensitivity / metabolism
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Hypersensitivity / physiopathology
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Inflammation / drug therapy*
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Inflammation / immunology
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Inflammation / metabolism
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Inflammation / physiopathology
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Interleukin-6 / biosynthesis
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Interleukin-8 / biosynthesis
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Male
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Mast Cells / cytology
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Mast Cells / drug effects
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Mast Cells / metabolism
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Mitogen-Activated Protein Kinase Kinases / genetics
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Mitogen-Activated Protein Kinase Kinases / metabolism
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Passive Cutaneous Anaphylaxis / drug effects*
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Passive Cutaneous Anaphylaxis / immunology
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Phosphorylation / drug effects
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Phytotherapy*
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Plant Extracts / therapeutic use
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Plant Roots / chemistry
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Rats
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Rats, Sprague-Dawley
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Tetradecanoylphorbol Acetate / pharmacology
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Tumor Necrosis Factor-alpha / biosynthesis
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p-Methoxy-N-methylphenethylamine / pharmacology
Substances
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Anti-Allergic Agents
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Interleukin-6
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Interleukin-8
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Plant Extracts
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Tumor Necrosis Factor-alpha
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Calcimycin
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p-Methoxy-N-methylphenethylamine
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Mitogen-Activated Protein Kinase Kinases
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Tetradecanoylphorbol Acetate