An epidemiologic surveillance of Shiga-like toxin-producing Escherichia coli infection in Argentinean children: risk factors and serum Shiga-like toxin 2 values

Pediatr Infect Dis J. 2012 Jan;31(1):20-4. doi: 10.1097/INF.0b013e31822ea6cf.

Abstract

Background and aims: Shiga-like toxin (Stx)-producing Escherichia coli (STEC) infection is an ongoing health issue that can lead to serious complications, including hemolytic uremic syndrome (HUS) and death. This study assessed demographic and epidemiologic information of STEC infection among Argentinean children.

Methods: A prospective surveillance of 2435 screened children (age, 0.5-15 years) presenting with watery diarrhea and/or bloody diarrhea was undertaken to evaluate the clinical course of STEC infection.

Results: Prevalence of STEC infection was 4.1% among subjects presenting with watery diarrhea for ≤ 5 days' duration, bloody diarrhea for ≤ 36 hours' duration, or both. Incidence of STEC infection was significantly higher in the subjects with bloody diarrhea. Ninety-three STEC+ children underwent further evaluation, of whom 8 (8.6%) developed HUS. White blood cells, particularly neutrophils, were abnormally elevated at screening in 5 of 8 HUS subjects. Quantifiable serum Stx-2 values were noted within 24 to 48 hours after the onset of bloody diarrhea in 3 HUS subjects using a validated chemiluminescence assay, with levels quickly dissipating by HUS onset.

Conclusions: Results suggest that young STEC-positive children with bloody diarrhea and exhibiting neutrophilic leukocytosis in the early course of their diarrhea are at risk for HUS progression. The observation of measurable concentrations of Stx-2 levels in the early post-bloody-diarrhea period and rapid dissipation at the time of HUS onset requires further evaluation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Argentina / epidemiology
  • Child
  • Diarrhea / diagnosis
  • Diarrhea / epidemiology*
  • Diarrhea / microbiology
  • Escherichia coli Infections / diagnosis
  • Escherichia coli Infections / epidemiology*
  • Escherichia coli Infections / microbiology
  • Female
  • Hemolytic-Uremic Syndrome / diagnosis
  • Hemolytic-Uremic Syndrome / epidemiology*
  • Hemolytic-Uremic Syndrome / microbiology
  • Humans
  • Infant
  • Male
  • Polymerase Chain Reaction
  • Population Surveillance / methods
  • Prevalence
  • Risk Factors
  • Shiga Toxin 2 / biosynthesis*
  • Shiga Toxin 2 / genetics
  • Shiga Toxins / biosynthesis*
  • Shiga Toxins / genetics
  • Shiga-Toxigenic Escherichia coli / isolation & purification*
  • Shiga-Toxigenic Escherichia coli / pathogenicity

Substances

  • Shiga Toxin 2
  • Shiga Toxins