Vascular endothelial growth factor-induced elimination of the type 1 interferon receptor is required for efficient angiogenesis

Blood. 2011 Oct 6;118(14):4003-6. doi: 10.1182/blood-2011-06-359745. Epub 2011 Aug 10.

Abstract

Angiogenesis is stimulated by vascular endothelial growth factor (VEGF) and antagonized by type 1 interferons, including IFN-α/β. On engaging their respective receptors (VEGFR2 and IFNAR), both stimuli activate protein kinase D2 (PKD2) and type 1 IFNs require PKD2 activation and recruitment to IFNAR1 to promote the phosphorylation-dependent ubiquitination, down-regulation, and degradation of the cognate receptor chain, IFNAR1. Data reveal that PKD2 activity is dispensable for VEGF-stimulated down-regulation of VEGFR2. Remarkably, VEGF treatment promotes the recruitment of PKD2 to IFNAR1 as well as ensuing phosphorylation, ubiquitination, and degradation of IFNAR1. In cells exposed to VEGF, phosphorylation-dependent degradation of IFNAR1 leads to an inhibition of type 1 IFN signaling and is required for efficient VEGF-stimulated angiogenesis. Importance of this mechanism for proangiogenic or antiangiogenic responses in cells exposed to counteracting stimuli and the potential medical significance of this regulation are discussed.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Cell Line
  • Endothelial Cells / cytology
  • Endothelial Cells / metabolism
  • Humans
  • Interferon Type I / metabolism
  • Interferon-alpha / metabolism
  • Mice
  • Neovascularization, Physiologic*
  • Phosphorylation
  • Protein Kinase D2
  • Protein Kinases / metabolism
  • Receptor, Interferon alpha-beta / metabolism*
  • Ubiquitination
  • Vascular Endothelial Growth Factor A / metabolism*
  • Vascular Endothelial Growth Factor Receptor-2 / metabolism

Substances

  • IFNAR1 protein, human
  • Interferon Type I
  • Interferon-alpha
  • Protein Kinase D2
  • Vascular Endothelial Growth Factor A
  • Receptor, Interferon alpha-beta
  • Protein Kinases
  • Vascular Endothelial Growth Factor Receptor-2