Influence of temperature and period of freezing on the generation of hemolysate and blood cell lysate

Giuseppe Lippi; Roberta Musa; Rosalia Aloe; Mariella Mercadanti; Silvia Pipitone

doi:10.1016/j.clinbiochem.2011.07.015

Influence of temperature and period of freezing on the generation of hemolysate and blood cell lysate

Clin Biochem. 2011 Oct;44(14-15):1267-9. doi: 10.1016/j.clinbiochem.2011.07.015. Epub 2011 Aug 6.

Authors

Giuseppe Lippi¹, Roberta Musa, Rosalia Aloe, Mariella Mercadanti, Silvia Pipitone

Affiliation

¹ U.O. Diagnostica Ematochimica, Dipartimento di Patologia e Medicina di Laboratorio, Azienda Ospedaliero-Universitaria di Parma, Italy. [email protected]

PMID: 21843520
DOI: 10.1016/j.clinbiochem.2011.07.015

Abstract

Background: There is little information on temperature and period of freezing for generating hemolysate and blood cell lysate.

Materials and methods: Primary tubes containing whole blood were frozen at -20°C and -80°C and then serially thawed to assess cell lysis and clinical chemistry tests in centrifuged plasma.

Results and conclusions: Massive amount of blood cells lysis could only be obtained by storing samples 12h at -20°C, or 2h at -80°C.

MeSH terms

Blood Cells*
Blood Preservation / methods*
Freezing
Humans
Temperature
Time Factors