Abstract
Cryptic unstable transcripts (CUTs) have been recently described as a major class of non-coding RNAs. These transcripts are, however, extremely unstable in normal cells and their analyzes pose specific technical problems. In this chapter, after a brief introduction discussing general aspects associated with the analysis of non-coding RNAs, we provide details of methods to enrich, map, and quantify this unconventional class of transcripts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Cryopreservation
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Gene Expression Profiling
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Genome, Fungal / genetics*
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Genomics / methods*
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Molecular Sequence Data
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Polyadenylation
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RNA Stability*
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RNA, Fungal / chemistry*
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RNA, Fungal / genetics*
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RNA, Fungal / isolation & purification
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RNA, Fungal / metabolism
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RNA, Untranslated / chemistry
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RNA, Untranslated / genetics*
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RNA, Untranslated / isolation & purification
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RNA, Untranslated / metabolism*
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Ribonucleoproteins / isolation & purification
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Ribonucleoproteins / metabolism
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Saccharomyces cerevisiae / cytology
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Saccharomyces cerevisiae / genetics
Substances
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RNA, Fungal
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RNA, Untranslated
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Ribonucleoproteins