Abstract
We report the identification of yan, an ETS-domain transcription factor belonging to the Drosophila epidermal growth factor receptor (DER) pathway, as an antagonist of the Wingless signalling pathway. We demonstrate that cells lacking yan function in the Drosophila eye show increased Wingless pathway activity, and inhibition of Wingless signalling in yan(-/-) cells rescues the yan mutant phenotype. Biochemical analysis shows that Yan physically associates with Armadillo, a crucial effector of the Wingless pathway, thereby suggesting a direct regulatory mechanism. We conclude that yan represents a new and unsuspected molecular link between the Wingless and DER pathways.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Armadillo Domain Proteins / antagonists & inhibitors
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Cell Line
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Drosophila / genetics
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Drosophila / metabolism*
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Drosophila Proteins / antagonists & inhibitors
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Drosophila Proteins / deficiency
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism*
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Eye / metabolism
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Eye Proteins / genetics
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Eye Proteins / metabolism*
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Gene Expression
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Gene Expression Regulation
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Gene Knockout Techniques
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Protein Binding
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Repressor Proteins / deficiency
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Signal Transduction*
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Transcription Factors / antagonists & inhibitors
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Wnt1 Protein / metabolism*
Substances
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AOP protein, Drosophila
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ARM protein, Drosophila
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Armadillo Domain Proteins
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Drosophila Proteins
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Eye Proteins
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Repressor Proteins
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Transcription Factors
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Wnt1 Protein
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wg protein, Drosophila