Fatal pulmonary thromboembolism. A retrospective autopsy study: searching for genetic thrombophilias (Factor V Leiden (G1691A) and FII (G20210A) gene variants) and dating the thrombus

Forensic Sci Int. 2012 Jan 10;214(1-3):152-8. doi: 10.1016/j.forsciint.2011.07.046. Epub 2011 Aug 26.

Abstract

The accuracy of antemortem diagnosis of pulmonary embolism is within the range of just 10-30%, so representing one of the most frequent missed diagnosis in sudden, unexpected death. We describe 43 fatal cases of pulmonary embolism as confirmed by post-mortem examination. The aim of our study was to verify the systematic search for the most common genetic thrombophilias (Factor V Leiden (G1691A) and FII (G20210A) gene variants) and dating the thrombus. As a whole, 41 patients (95.3%) had at least one risk factor. Pre-existing symptoms are described just before fatal embolism in 18 (41.9%) out 43 patients. In 18 out of 43 (41.9%) it was not possible to find the thrombotic site. In 24 out of the remaining 25 cases the involvement of the deep veins of one leg was shown; in 1 case the thrombus was localised in the inferior caval vein. 10 (41.7%) were iliac vein thromboses, 7 (29.1%) femoral, 2 (8.3%) popliteal, 3 (12.6%) posterior-tibial, 1 (4.1%) anterior-tibial and 1 (4.1%) peroneal vein thromboses. In our cohort of patients, 4 (10%) out of 40 cases carried the 20210A prothrombin gene variant in heterozygosis. One (2.5%) out of 40 carried the Factor V Leiden (G1691A) gene variant in heterozygosis. Patients carrying these gene variants in homozygosis or carrying both were not present in our case-series. We strongly underline the relevance of a complete methodological approach, integrating clinical data by means of autopsy findings and histological study. On the contrary, investigating common inherited thrombophilia is not warranted.

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Factor V / genetics*
  • Female
  • Forensic Pathology
  • Heterozygote
  • Humans
  • Male
  • Middle Aged
  • Polymorphism, Genetic
  • Prothrombin / genetics*
  • Pulmonary Embolism / pathology*
  • Retrospective Studies
  • Risk Factors
  • Venous Thrombosis / genetics
  • Venous Thrombosis / pathology*
  • Young Adult

Substances

  • factor V Leiden
  • Factor V
  • Prothrombin