Abstract
Objective:
The aim of this study was to assess the TOP2A RNA expression and the relationship of TOP2A protein expression with metastasis-free interval in breast cancer patients.
Methods:
TOP2A expression was analyzed prior to surgery in 86 patients. The level of TOP2A gene amplification was analyzed by fluorescence in situ hybridization (FISH), its RNA expression level with RT-PCR, and their correlation with TOP2A protein expression was assessed by immunohistochemistry (IHC). The correlation between RNA expression level and metastasis-free interval in breast cancer patients was also analyzed.
Results:
Aberrations (amplification or deletion) of TOP2A copy number was observed in 25.6% (22/86) of the cases. TOP2A protein expression was detected in 66.3% (57/86) of the samples. There was a significant correlation between the TOP2A RNA expression and protein expression (P < 0.001). TOP2A gene expression was significantly associated with the metastasis-free interval in the breast cancer patients (P = 0.001). There was no significant correlation between TOP2A gene amplification and TOP2A protein expression (P = 0.211).
Conclusions:
TOP2A RNA level is an objective and reliable prognostic indicator in breast cancer.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Neoplasm / genetics
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Antigens, Neoplasm / metabolism*
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Breast Neoplasms / drug therapy
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Breast Neoplasms / genetics*
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Breast Neoplasms / metabolism
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Breast Neoplasms / surgery
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Carcinoma, Ductal, Breast / drug therapy
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Carcinoma, Ductal, Breast / genetics*
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Carcinoma, Ductal, Breast / metabolism
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Carcinoma, Ductal, Breast / surgery
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Carcinoma, Intraductal, Noninfiltrating / drug therapy
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Carcinoma, Intraductal, Noninfiltrating / genetics
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Carcinoma, Intraductal, Noninfiltrating / metabolism
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Carcinoma, Intraductal, Noninfiltrating / surgery
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Carcinoma, Lobular / drug therapy
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Carcinoma, Lobular / genetics
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Carcinoma, Lobular / metabolism
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Carcinoma, Lobular / surgery
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Chemotherapy, Adjuvant
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DNA Topoisomerases, Type II / genetics
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DNA Topoisomerases, Type II / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Disease-Free Survival
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Female
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Gene Amplification
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Gene Deletion
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Gene Expression Regulation, Neoplastic
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Humans
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Middle Aged
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Neoadjuvant Therapy
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Poly-ADP-Ribose Binding Proteins
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RNA / metabolism
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Remission Induction
Substances
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Antigens, Neoplasm
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DNA-Binding Proteins
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Poly-ADP-Ribose Binding Proteins
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RNA
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DNA Topoisomerases, Type II
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TOP2A protein, human