The vaccines of the Expanded Immunization Program are administered free of charge to beneficiaries. However, these vaccines are purchased by countries and partners of immunization. These costs need to be estimated to be better understood. We conducted a descriptive cross-sectional study of the costs of the Expanded Immunization Program in the health district of Grand-Bassam from January 1 to December 31, 2006, with questions aiming to understand the costs from the point of view of the state and partners. We aimed to determine costs by level of expenditure, calculate the cost per child who received 3 doses of vaccine against Diphtheria-Tetanus-Pertussis-Hepatitis B and cost per strategy. Vaccines and injection supplies accounted for 49% of recurrent costs. Vehicles and motorcycles for transport accounted for 73% of non-recurrent costs. The recurrent cost per child who received 3 doses of the vaccine was 10 797 FCFA (16 euros). The recurrent cost per dose administered was 1,041 FCFA (1,58 euros) for the fixed strategy, 4,232 FCFA (6,45 euros) for the outreach strategy and 4,058 FCFA (6,18 euros) for the mobile strategy. Because of the scarcity of financial resources, the Côte d'Ivoire government must strengthen efficient public-health measures, including vaccination.