Abstract
Transcriptional responses to extracellular stimuli involve tuning the rates of transcript production and degradation. Here, we show that the time-dependent profiles of these rates can be inferred from simultaneous measurements of precursor mRNA (pre-mRNA) and mature mRNA profiles. Transcriptome-wide measurements demonstrate that genes with similar mRNA profiles often exhibit marked differences in the amplitude and onset of their production rate. The latter is characterized by a large dynamic range, with a group of genes exhibiting an unexpectedly strong transient production overshoot, thereby accelerating their induction and, when combined with time-dependent degradation, shaping transient responses with precise timing and amplitude.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptation, Biological
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Animals
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Cell Line
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DNA Probes / analysis
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Dendritic Cells / cytology
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Dendritic Cells / drug effects
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Dendritic Cells / metabolism*
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Epidermal Growth Factor / pharmacology
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Female
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Genomics*
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Humans
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Lipopolysaccharides / pharmacology
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Mammary Glands, Human / cytology
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Mammary Glands, Human / drug effects
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Mammary Glands, Human / metabolism*
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Mice
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Mice, Inbred C57BL
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Models, Statistical
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RNA Precursors* / genetics
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RNA Precursors* / metabolism
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RNA Stability*
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RNA, Messenger* / biosynthesis
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RNA, Messenger* / genetics
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Real-Time Polymerase Chain Reaction
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Stimulation, Chemical
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Time Factors
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Transcription, Genetic*
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Transcriptome / genetics*
Substances
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DNA Probes
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Lipopolysaccharides
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RNA Precursors
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RNA, Messenger
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Epidermal Growth Factor