Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe

Haibin Shi; Xiamin Cheng; Siu Kwan Sze; Shao Q Yao

doi:10.1039/c1cc14824a

Proteome profiling reveals potential cellular targets of staurosporine using a clickable cell-permeable probe

Chem Commun (Camb). 2011 Oct 28;47(40):11306-8. doi: 10.1039/c1cc14824a. Epub 2011 Sep 16.

Authors

Haibin Shi¹, Xiamin Cheng, Siu Kwan Sze, Shao Q Yao

Affiliation

¹ Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543.

PMID: 21922114
DOI: 10.1039/c1cc14824a

Abstract

A clickable, affinity-based probe (AfBP), which was modified from staurosporine (a natural product kinase inhibitor), has been synthesized and used in situ for activity-based proteome profiling of potential cellular targets of staurosporine in HepG2 cancer cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Click Chemistry*
Hep G2 Cells
Humans
Molecular Probes / chemical synthesis
Molecular Probes / chemistry*
Molecular Probes / metabolism*
Permeability
Protein Kinase Inhibitors / metabolism*
Protein Kinases / metabolism
Proteomics / methods*
Staurosporine / metabolism*

Substances

Molecular Probes
Protein Kinase Inhibitors
Protein Kinases
Staurosporine