Bradykinin and des-Arg10-kallidin enhance the adhesion of polymorphonuclear leukocytes to extracellular matrix proteins and endothelial cells

Cell Commun Adhes. 2011 Aug;18(4):67-71. doi: 10.3109/15419061.2011.617854. Epub 2011 Sep 26.

Abstract

Bradykinin-related peptides (kinins) are well known to contribute to leukocyte recruitment to inflammatory foci; however, a role of these universal pro-inflammatory mediators in the first step of this process, i.e. the leukocyte adhesion to endothelial cells, is not well understood. In this work we found that bradykinin and des-Arg10-kallidin enhance the adhesion of polymorphonuclear bloods cells (PMN) to fibrinogen and fibronectin. Also, the PMN adherence to endothelial cells of HMEC-1 line strongly increased after stimulation by kinins, particularly des-Arg10-kallidin, or when PMN were co-stimulated with bradykinin and interleukin-1β. These effects were attenuated after PMN treatment with a specific inhibitor of carboxypeptidases, which convert kinins to their des-Arg metabolites. The kinin peptides were also able to change the Mac-1 integrin expression on the PMN surface. These results suggest a regulatory effect of kinins on leukocyte adhesion to endothelial wall, providing new aspects of the leukocyte infiltration into inflamed tissues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bradykinin / pharmacology*
  • Carboxypeptidases / antagonists & inhibitors
  • Carboxypeptidases / metabolism
  • Cell Adhesion / drug effects
  • Cells, Cultured
  • Endothelial Cells / drug effects*
  • Extracellular Matrix Proteins / metabolism*
  • Humans
  • Interleukin-1beta / pharmacology
  • Kallidin / pharmacology*
  • Macrophage-1 Antigen / metabolism
  • Neutrophils / drug effects*
  • Neutrophils / physiology
  • Vasodilator Agents / pharmacology

Substances

  • Extracellular Matrix Proteins
  • Interleukin-1beta
  • Macrophage-1 Antigen
  • Vasodilator Agents
  • Kallidin
  • Carboxypeptidases
  • Bradykinin