A regression model approach to enable cell morphology correction in high-throughput flow cytometry

Mol Syst Biol. 2011 Sep 27:7:531. doi: 10.1038/msb.2011.64.

Abstract

Cells exposed to stimuli exhibit a wide range of responses ensuring phenotypic variability across the population. Such single cell behavior is often examined by flow cytometry; however, gating procedures typically employed to select a small subpopulation of cells with similar morphological characteristics make it difficult, even impossible, to quantitatively compare cells across a large variety of experimental conditions because these conditions can lead to profound morphological variations. To overcome these limitations, we developed a regression approach to correct for variability in fluorescence intensity due to differences in cell size and granularity without discarding any of the cells, which gating ipso facto does. This approach enables quantitative studies of cellular heterogeneity and transcriptional noise in high-throughput experiments involving thousands of samples. We used this approach to analyze a library of yeast knockout strains and reveal genes required for the population to establish a bimodal response to oleic acid induction. We identify a group of epigenetic regulators and nucleoporins that, by maintaining an 'unresponsive population,' may provide the population with the advantage of diversified bet hedging.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Size
  • Epigenomics*
  • Flow Cytometry* / methods
  • Flow Cytometry* / statistics & numerical data
  • Fluorescence
  • Genetic Variation
  • Glucose / metabolism
  • Glucose / pharmacology
  • Green Fluorescent Proteins / analysis
  • High-Throughput Screening Assays*
  • Models, Statistical*
  • Mutation
  • Nuclear Pore Complex Proteins / genetics
  • Nuclear Pore Complex Proteins / metabolism
  • Oleic Acid / metabolism
  • Oleic Acid / pharmacology
  • Organisms, Genetically Modified / genetics
  • Organisms, Genetically Modified / metabolism
  • Saccharomyces cerevisiae / cytology*
  • Saccharomyces cerevisiae / drug effects
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Telomere-Binding Proteins / genetics
  • Telomere-Binding Proteins / metabolism
  • Transcription, Genetic / drug effects*

Substances

  • Nuclear Pore Complex Proteins
  • Telomere-Binding Proteins
  • Green Fluorescent Proteins
  • Oleic Acid
  • Glucose