Abstract
A bilayer matrix consisting of TABP-SS/DNA complexes and sodium alginate gel is formed via electrostatic interaction. In vitro cell adhesion, proliferation and transfection of the bilayer matrix are investigated in HepG2, HeLa and COS7 cells. Results show that this matrix can only promote tumor cell attachment and growth. Compared with normal cells, the bilayer matrix exhibits significantly higher transfection efficacy in tumor cells. Cell co-culture competitive transfection assay shows that the cell uptake of TABP-SS/DNA complexes is significantly enhanced in tumor cells rather than normal cells under the co-culture competitive condition, which confirms that TABP-SS/DNA complexes have strong tumor cell selectivity and tumor targeting transfection ability.
Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alginates / chemistry
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Animals
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Avidin / chemistry
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Avidin / metabolism
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Biotin / chemistry
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Biotin / metabolism
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COS Cells
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Chlorocebus aethiops
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Coculture Techniques
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DNA / chemistry
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DNA / metabolism
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Drug Carriers / chemical synthesis*
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Drug Carriers / pharmacology
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Gels
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Glucuronic Acid / chemistry
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HeLa Cells
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Hep G2 Cells
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Hexuronic Acids / chemistry
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Humans
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Luciferases / analysis
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Molecular Targeted Therapy / methods*
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Neoplasms / pathology
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Neoplasms / therapy*
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Plasmids / chemistry
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Plasmids / metabolism
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Polyamines / chemistry
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Polyelectrolytes
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Polyethyleneimine / chemistry
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Receptors, Transferrin / chemistry
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Receptors, Transferrin / metabolism
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Static Electricity
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Transfection / methods*
Substances
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Alginates
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Drug Carriers
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Gels
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Hexuronic Acids
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Polyamines
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Polyelectrolytes
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Receptors, Transferrin
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polycations
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Avidin
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Biotin
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Glucuronic Acid
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Polyethyleneimine
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DNA
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Luciferases