ICSH recommendations for the measurement of haemoglobin A2

Int J Lab Hematol. 2012 Feb;34(1):1-13. doi: 10.1111/j.1751-553X.2011.01368.x. Epub 2011 Oct 5.

Abstract

Although DNA analysis is needed for characterization of the mutations that cause β-thalassaemia, measurement of the Hb A(2) is essential for the routine identification of people who are carriers of β-thalassaemia. The methods of quantitating Hb A(2) are described together with pitfalls in undertaking these laboratory tests with particular emphasis on automated high-performance liquid chromatography and capillary electrophoresis.

Publication types

  • Practice Guideline

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Electrophoresis, Capillary
  • Hemoglobin A2 / analysis*
  • Heterozygote
  • Humans
  • Reference Values
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Specimen Handling / methods
  • Terminology as Topic
  • beta-Thalassemia / diagnosis*
  • beta-Thalassemia / genetics

Substances

  • Hemoglobin A2