C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface

Eunjoo Kim; Hyun-Chul Kim; Se Geun Lee; Sung Jun Lee; Tae-Jung Go; Chul Su Baek; Sang Won Jeong

doi:10.1039/c1cc15079k

C-Reactive protein-directed immobilization of phosphocholine ligands on a solid surface

Chem Commun (Camb). 2011 Nov 21;47(43):11900-2. doi: 10.1039/c1cc15079k. Epub 2011 Oct 6.

Authors

Eunjoo Kim¹, Hyun-Chul Kim, Se Geun Lee, Sung Jun Lee, Tae-Jung Go, Chul Su Baek, Sang Won Jeong

Affiliation

¹ Nano & Bio Research Division, Daegu Gyeongbuk Institute of Science and Technology (DGIST), Daegu 711-873, Korea.

PMID: 21975508
DOI: 10.1039/c1cc15079k

Abstract

The complexes of C-reactive protein (CRP) with its polymerizable phosphocholine ligands adsorbed at the styrene-water interface were polymerized. The molecularly imprinted polymer (MIP) exhibited a binding affinity for CRP comparable to that of immobilized anti-CRP antibody. The determination of human serum CRP using the MIP-based sandwich immunoassay has been demonstrated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Immobilized / immunology
C-Reactive Protein / analysis*
C-Reactive Protein / immunology
Humans
Immunoassay / methods*
Ligands
Molecular Imprinting
Oils / chemistry
Phosphorylcholine / chemistry*
Polystyrenes / chemistry
Surface Properties
Water / chemistry

Substances

Antibodies, Immobilized
Ligands
Oils
Polystyrenes
Water
Phosphorylcholine
C-Reactive Protein