ICESp2905, the erm(TR)-tet(O) element of Streptococcus pyogenes, is formed by two independent integrative and conjugative elements

Antimicrob Agents Chemother. 2012 Jan;56(1):591-4. doi: 10.1128/AAC.05352-11. Epub 2011 Oct 10.

Abstract

In ICESp2905, a widespread erm(TR)- and tet(O)-carrying genetic element of Streptococcus pyogenes, the two resistance determinants are contained in separate fragments inserted into a scaffold of clostridial origin. ICESp2905 (∼65.6 kb) was transferable not only in its regular form but also in a defective form lacking the erm(TR) fragment (ICESp2906, ∼53.0 kb). The erm(TR) fragment was also an independent integrative and conjugative element (ICE) (ICESp2907, ∼12.6 kb). ICESp2905 thus results from one ICE (ICESp2907) being integrated into another (ICESp2906).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / administration & dosage
  • Base Sequence
  • Chromosome Mapping
  • Chromosomes, Bacterial
  • Conjugation, Genetic
  • DNA, Bacterial
  • DNA, Circular
  • Erythromycin / administration & dosage*
  • Genes, Bacterial*
  • Humans
  • Microbial Sensitivity Tests
  • Molecular Sequence Data
  • Open Reading Frames
  • Sequence Analysis, DNA
  • Streptococcal Infections / drug therapy*
  • Streptococcal Infections / microbiology
  • Streptococcal Infections / transmission
  • Streptococcus pyogenes / drug effects
  • Streptococcus pyogenes / genetics*
  • Streptococcus pyogenes / pathogenicity
  • Tetracycline / administration & dosage*
  • Tetracycline Resistance*

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • DNA, Circular
  • Erythromycin
  • Tetracycline