Structure of the O-Specific polysaccharide from Shewanella japonica KMM 3601 containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid

E L Nazarenko; A V Perepelov; L S Shevchenko; E D Daeva; E P Ivanova; A S Shashkov; G Widmalm

doi:10.1134/S0006297911070091

Structure of the O-Specific polysaccharide from Shewanella japonica KMM 3601 containing 5,7-Diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid

Biochemistry (Mosc). 2011 Jul;76(7):791-6. doi: 10.1134/S0006297911070091.

Authors

E L Nazarenko¹, A V Perepelov, L S Shevchenko, E D Daeva, E P Ivanova, A S Shashkov, G Widmalm

Affiliation

¹ Paciﬁc Institute of Bioorganic Chemistry, Far East Branch of the Russian Academy of Sciences, Vladivostok, Russia.

PMID: 21999540
DOI: 10.1134/S0006297911070091

Abstract

Structure of the O-specific polysaccharide chain of the lipopolysaccharide (LPS) of Shewanella japonica KMM 3601 was elucidated. The initial and O-deacylated LPS as well as a trisaccharide representing the O-deacetylated repeating unit of the O-specific polysaccharide were studied by sugar analysis along with 1H and 13C NMR spectroscopy. The polysaccharide was found to contain a rare higher sugar, 5,7-diacetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-non-2-ulosonic acid (a derivative of 4-epilegionaminic acid, 4eLeg). The following structure of the trisaccharide repeating unit was established: →4)-α-4eLegp5Ac7Ac-(2→4)-β-D-GlcpA3Ac-(1→3)-β-D-GalpNAc-(1→.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Molecular Sequence Data
Nuclear Magnetic Resonance, Biomolecular
O Antigens / chemistry*
Shewanella / chemistry*
Shewanella / immunology
Sugar Acids / analysis

Substances

5,7-diacetamido-3,5,7,9-tetradeoxynonulosonic acid
O Antigens
Sugar Acids