Monitoring the active transport of efflux pumps after their reconstitution into proteoliposomes: caveats and keys

M Picard; A Verchère; I Broutin

doi:10.1016/j.ab.2011.09.025

Monitoring the active transport of efflux pumps after their reconstitution into proteoliposomes: caveats and keys

Anal Biochem. 2012 Jan 15;420(2):194-6. doi: 10.1016/j.ab.2011.09.025. Epub 2011 Oct 2.

Authors

M Picard¹, A Verchère, I Broutin

Affiliation

¹ Laboratoire de Cristallographie et RMN Biologiques (CNRS UMR 8015), Faculté de Pharmacie, Université Paris Descartes, Sorbonne Paris Cité 75006 Paris, France. [email protected]

PMID: 22019764
DOI: 10.1016/j.ab.2011.09.025

Abstract

There is an acute need for a functional assay allowing the investigation of efflux pumps. A dedicated procedure was previously developed, but although it was unambiguous, it suffered from a lack of reproducibility. We describe an optimization of the procedure that makes the assay much more robust.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arylsulfonates / metabolism
Biological Assay / methods*
Biological Transport / drug effects
Hydrogen-Ion Concentration
Liposomes / chemistry
Liposomes / metabolism
Proteolipids / metabolism*
Valinomycin / pharmacology

Substances

Arylsulfonates
Liposomes
Proteolipids
proteoliposomes
Valinomycin
pyranine