Abstract
NKX2-5 is expressed in the heart throughout life. We targeted eGFP sequences to the NKX2-5 locus of human embryonic stem cells (hESCs); NKX2-5(eGFP/w) hESCs facilitate quantification of cardiac differentiation, purification of hESC-derived committed cardiac progenitor cells (hESC-CPCs) and cardiomyocytes (hESC-CMs) and the standardization of differentiation protocols. We used NKX2-5 eGFP(+) cells to identify VCAM1 and SIRPA as cell-surface markers expressed in cardiac lineages.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antigens, Differentiation / genetics
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Antigens, Differentiation / metabolism
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Biomarkers / analysis
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Cell Differentiation
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Cell Separation / methods*
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Embryonic Stem Cells / cytology*
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Embryonic Stem Cells / metabolism*
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Gene Expression Profiling
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Green Fluorescent Proteins / metabolism*
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Homeobox Protein Nkx-2.5
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism*
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Humans
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Myoblasts, Cardiac / cytology*
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Myoblasts, Cardiac / metabolism
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Myocytes, Cardiac / cytology*
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Myocytes, Cardiac / metabolism
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Polymerase Chain Reaction
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Receptors, Immunologic / genetics
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Receptors, Immunologic / metabolism
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Vascular Cell Adhesion Molecule-1 / genetics
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Vascular Cell Adhesion Molecule-1 / metabolism
Substances
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Antigens, Differentiation
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Biomarkers
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Homeobox Protein Nkx-2.5
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Homeodomain Proteins
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NKX2-5 protein, human
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Receptors, Immunologic
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SIRPA protein, human
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Transcription Factors
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Vascular Cell Adhesion Molecule-1
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Green Fluorescent Proteins