Abstract
Deep tissue in vivo two-photon fluorescence imaging of cortical vasculature in a mouse brain using 1280-nm excitation is presented. A record imaging depth of 1.6 mm in mouse cortex is achieved in vivo, approximately reaching the fundamental depth limit in scattering tissue.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Cerebral Cortex / anatomy & histology*
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Cerebral Cortex / blood supply*
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Cerebrovascular Circulation
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Fluorescent Dyes / administration & dosage
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Imaging, Three-Dimensional / methods
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Male
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Mice
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Microscopy, Fluorescence, Multiphoton / methods*
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Optical Phenomena