Heart failure decreases nerve activity in the right atrial ganglionated plexus

J Cardiovasc Electrophysiol. 2012 Apr;23(4):404-12. doi: 10.1111/j.1540-8167.2011.02204.x. Epub 2011 Oct 28.

Abstract

Objective: We tested the hypothesis that heart failure (HF) results in right atrial ganglionated plexus (RAGP) denervation that contributes to sinoatrial node dysfunction.

Background: HF is associated with sinoatrial node dysfunction. However, the detailed mechanisms remain unclear.

Methods: We recorded nerve activity (NA) from the RAGP, right stellate ganglion (SG), and right vagal nerve in 7 ambulatory dogs at baseline and after pacing-induced HF. We also determined the effects of RAGP stimulation in isolated normal and HF canine RA.

Results: NAs in both the SG and vagal were significantly higher in HF than at baseline. The relationship between 1-minute integrated NAs of vagal and RAGP showed either a positive linear correlation (Group 1, n = 4) or an L-shaped correlation (Group 2, n = 3). In all dogs, a reduced heart rate was observed when vagal-NA was associated with simultaneously increased RAGP-NA. On the other hand, when vagal-NA was not associated with increased RAGP-NA, the heart rate was not reduced. The induction of HF significantly decreased RAGP-NA in all dogs (P < 0.05). Stimulating the superior RAGP in isolated RA significantly reduced the sinus rate in normal but not the HF hearts. Immunohistochemical staining revealed lower densities of tyrosine hydroxylase- and choline acetyltransferase-positive nerve tissues in HF RAGP than normal (P < 0.001 and P = 0.001, respectively).

Conclusions: The RAGP-NA is essential for the vagal nerve to counterbalance the SG in sinus rate control. In HF, RAGP denervation and decreased RAGP-NA contribute to the sinus node dysfunction.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials
  • Animals
  • Cardiac Pacing, Artificial
  • Choline O-Acetyltransferase / metabolism
  • Disease Models, Animal
  • Dogs
  • Electrocardiography
  • Heart Atria / innervation
  • Heart Failure / etiology
  • Heart Failure / metabolism
  • Heart Failure / physiopathology*
  • Heart Rate*
  • Immunohistochemistry
  • Male
  • Sinoatrial Node / innervation*
  • Stellate Ganglion / enzymology
  • Stellate Ganglion / physiopathology*
  • Time Factors
  • Tyrosine 3-Monooxygenase / metabolism
  • Vagus Nerve / enzymology
  • Vagus Nerve / physiopathology*
  • Voltage-Sensitive Dye Imaging

Substances

  • Tyrosine 3-Monooxygenase
  • Choline O-Acetyltransferase