Genetic evidence for preferential strand transfer during meiotic recombination in yeast

Genetics. 1990 Aug;125(4):753-61. doi: 10.1093/genetics/125.4.753.

Abstract

During meiotic recombination in the yeast Saccharomyces cerevisiae, heteroduplexes are formed as an intermediate in the exchange process. In the formation of an asymmetric heteroduplex, one chromosome acts as a donor of a single DNA strand and the other acts as a recipient. We present genetic evidence that the nontranscribed strand is donated more frequently than the transcribed strand in spores that have an unrepaired mismatch at the HIS4 locus.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Repair
  • DNA, Fungal / genetics
  • DNA, Fungal / metabolism
  • Genes, Fungal
  • Histidine / metabolism
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Nucleic Acid Heteroduplexes / metabolism
  • Recombination, Genetic*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / physiology
  • Spores, Fungal
  • Transcription, Genetic*

Substances

  • DNA, Fungal
  • Nucleic Acid Heteroduplexes
  • Histidine