Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging

Ginni Grover; Sean Quirin; Callie Fiedler; Rafael Piestun

doi:10.1364/BOE.2.003010

Photon efficient double-helix PSF microscopy with application to 3D photo-activation localization imaging

Biomed Opt Express. 2011 Nov 1;2(11):3010-20. doi: 10.1364/BOE.2.003010. Epub 2011 Oct 5.

Authors

Ginni Grover¹, Sean Quirin, Callie Fiedler, Rafael Piestun

Affiliation

¹ Department of Electrical, Computer, and Energy Engineering, University of Colorado, Boulder, CO 80309, USA.

Abstract

We present a double-helix point spread function (DH-PSF) based three-dimensional (3D) microscope with efficient photon collection using a phase mask fabricated by gray-level lithography. The system using the phase mask more than doubles the efficiency of current liquid crystal spatial light modulator implementations. We demonstrate the phase mask DH-PSF microscope for 3D photo-activation localization microscopy (PM-DH-PALM) over an extended axial range.

Keywords: (050.1970) Diffractive optics; (110.4850) Optical transfer functions; (180.6900) Three-dimensional microscopy; (220.4000) Microstructure fabrication.