Following G-quadruplex formation by its intrinsic fluorescence

Nguyen Thuan Dao; Reinhard Haselsberger; Maria-Elisabeth Michel-Beyerle; Anh Tuân Phan

doi:10.1016/j.febslet.2011.11.004

Following G-quadruplex formation by its intrinsic fluorescence

FEBS Lett. 2011 Dec 15;585(24):3969-77. doi: 10.1016/j.febslet.2011.11.004. Epub 2011 Nov 10.

Authors

Nguyen Thuan Dao¹, Reinhard Haselsberger, Maria-Elisabeth Michel-Beyerle, Anh Tuân Phan

Affiliation

¹ Division of Physics and Applied Physics, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore.

PMID: 22079665
DOI: 10.1016/j.febslet.2011.11.004

Abstract

We characterized and compared the fluorescence properties of various well-defined G-quadruplex structures. The increase of intrinsic fluorescence of G-rich DNA sequences when they form G-quadruplexes can be used to monitor the folding and unfolding of G-quadruplexes as a function of cations and temperature. The temperature-dependent fluorescence spectra of different G-quadruplexes also exhibit characteristic patterns. Thus, the stability and possibly also the structure of G-quadruplexes can be characterized and distinguished by their intrinsic fluorescence spectra.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA, Z-Form / chemistry
G-Quadruplexes*
Giardia / genetics
Humans
Oxytricha / genetics
Reproducibility of Results
Spectrometry, Fluorescence
Telomere / chemistry
Telomere / genetics
Temperature
Time Factors

Substances

DNA, Z-Form