Fruit and vegetable and fried food consumption and 3-(2-deoxy-β-D-erythro-pentafuranosyl)pyrimido[1,2-α] purin-10(3H)-one deoxyguanosine adduct formation

Free Radic Res. 2012 Jan;46(1):85-92. doi: 10.3109/10715762.2011.640676. Epub 2011 Dec 1.

Abstract

Diet has been shown to modulate M(1)dG adduct, a biomarker of oxidative stress and lipid peroxidation. Thus, we analysed the association between diet and M(1)dG in 120 controls and 67 Map Ta Phut industrial estate workers in Rayong, Thailand, to evaluate the influence of fruit and vegetables, and fried and charcoal-grilled/barbecued food consumption on M(1)dG. M(1)dG was decreased in controls reporting to consume 14-17 servings/week of fruit and vegetables (mean ratio [MR]= 0.35, CI 0.18-0.69, p< 0.05). Conversely, a non-statistically significant M(1)dG increment was detected in controls consuming 9-18 servings/week of fried food (MR = 1.33, CI 0.88-2.00, p = 0.168). No effect of charcoal-grilled/barbecued food was found. No effect of diet was observed in workers. An association with smoking was observed in controls (MR = 1.88, CI 1.14-3.10, p < 0.05), but not in workers. M(1)dG can induce mutations and/or methylation changes within the promoter regions of cancer-related genes, thus promotion of healthy eating practices should be recommended.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • DNA / blood
  • DNA / metabolism
  • DNA Adducts / biosynthesis
  • DNA Adducts / blood
  • DNA Adducts / metabolism*
  • Deoxyguanosine / biosynthesis
  • Deoxyguanosine / blood
  • Deoxyguanosine / metabolism*
  • Diet*
  • Feeding Behavior
  • Female
  • Fruit*
  • Humans
  • Lipid Peroxidation
  • Male
  • Malondialdehyde / blood
  • Mass Spectrometry
  • Oxidative Stress
  • Purine Nucleosides / metabolism*
  • Vegetables*

Substances

  • 3-(2'-deoxy-beta-D-erythro-pentofuranosyl)pyrimido(1,2-alpha)purin-10(3H)-one
  • DNA Adducts
  • Purine Nucleosides
  • Malondialdehyde
  • DNA
  • calf thymus DNA
  • Deoxyguanosine