Genistein reduces heparan sulfate accumulation in human mucolipidosis II skin fibroblasts

Takanobu Otomo; Mohammad Arif Hossain; Keiichi Ozono; Norio Sakai

doi:10.1016/j.ymgme.2011.10.015

Genistein reduces heparan sulfate accumulation in human mucolipidosis II skin fibroblasts

Mol Genet Metab. 2012 Feb;105(2):266-9. doi: 10.1016/j.ymgme.2011.10.015. Epub 2011 Oct 30.

Authors

Takanobu Otomo¹, Mohammad Arif Hossain, Keiichi Ozono, Norio Sakai

Affiliation

¹ Department of Pediatrics, Osaka University Graduate School of Medicine, 2-2 Yamada-oka, Suita, Osaka 565-0871, Japan. [email protected]

PMID: 22088809
DOI: 10.1016/j.ymgme.2011.10.015

Abstract

Genistein, a soy isoflavone, reduces glycosaminoglycan synthesis and its effect on mucopolysaccharidoses has been tested. In this report, we examined the effect of genistein in human mucolipidosis II skin fibroblasts in vitro. Heparan sulfate was accumulated within both cells and in extracellular spaces in mucolipidosis II. Genistein reduced the amount of heparan sulfate in cultured cells dose dependently and also inhibited cell growth dose dependently.

MeSH terms

Antibodies, Anti-Idiotypic / immunology
Cells, Cultured
Dose-Response Relationship, Drug
Extracellular Space / drug effects
Extracellular Space / metabolism*
Fibroblasts / drug effects
Fibroblasts / metabolism*
Genistein / pharmacology*
Heparitin Sulfate / immunology
Heparitin Sulfate / isolation & purification
Heparitin Sulfate / metabolism*
Humans
Inclusion Bodies / drug effects
Inclusion Bodies / metabolism
Mucolipidoses / metabolism*
Skin / metabolism

Substances

Antibodies, Anti-Idiotypic
Heparitin Sulfate
Genistein