Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia

Sam Abraham; James Chin; Huub J M Brouwers; Ren Zhang; Toni A Chapman

doi:10.1016/j.mimet.2011.10.016

Molecular serogrouping of porcine enterotoxigenic Escherichia coli from Australia

J Microbiol Methods. 2012 Jan;88(1):73-6. doi: 10.1016/j.mimet.2011.10.016. Epub 2011 Nov 9.

Authors

Sam Abraham¹, James Chin, Huub J M Brouwers, Ren Zhang, Toni A Chapman

Affiliation

¹ Microbiological Diseases and Diagnostics Research, New South Wales Department of Primary Industries, Elizabeth Macarthur Agricultural Institute, Menangle, NSW, 2568, Australia.

PMID: 22093999
DOI: 10.1016/j.mimet.2011.10.016

Abstract

Enterotoxigenic Escherichia coli (ETEC) is a common etiological agent of neonatal, pre and post weaning diarrhoea in piglets. One of the most important steps in the diagnosis and epidemiological understanding of this organism is accurate serogrouping. In many instances, however, conventional serogrouping fails to produce accurate identification of serogroups. In this communication we report a modified and simplified molecular serogrouping method (rfb-RFLP) for the accurate identification of the most common porcine ETEC strains that cause neonatal, pre and post weaning diarrhoea in Australia.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Amplified Fragment Length Polymorphism Analysis / methods*
Animals
Australia
Bacterial Typing Techniques / methods*
Enterotoxigenic Escherichia coli / classification
Enterotoxigenic Escherichia coli / genetics*
Enterotoxigenic Escherichia coli / isolation & purification*
Escherichia coli Infections / diagnosis
Escherichia coli Infections / microbiology
Escherichia coli Infections / veterinary*
Phylogeny
Polymorphism, Restriction Fragment Length
Swine
Swine Diseases / diagnosis
Swine Diseases / microbiology*