A FlAsH-based cross-linker to study protein interactions in living cells

Angew Chem Int Ed Engl. 2011 Dec 23;50(52):12655-8. doi: 10.1002/anie.201106404. Epub 2011 Nov 16.

Abstract

As you like it: xCrAsH, a dimeric derivative of the arsenical compound FlAsH, enables the highly specific, covalent cross-linking of two proteins containing a 12 amino acid peptide tag. This inducible and (by addition of dithiols) reversible system can be used to detect and manipulate protein-protein interactions both in vitro and in living cells (see picture).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arsenicals / chemistry*
  • Cross-Linking Reagents / chemistry*
  • Fluoresceins / chemistry*
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Proteins / chemistry*

Substances

  • Arsenicals
  • Cross-Linking Reagents
  • Fluoresceins
  • Fluorescent Dyes
  • Proteins