Crystallization and preliminary crystallographic studies of Campylobacter jejuni ChuZ, a member of a novel haem oxygenase family

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Oct 1;67(Pt 10):1228-30. doi: 10.1107/S1744309111026194. Epub 2011 Sep 29.

Abstract

The haem oxygenase ChuZ from Campylobacter jejuni, a major enteric pathogen in humans, is part of the iron-acquisition mechanism that is involved in bacterial survival and persistence in hosts. The ChuZ-haemin complex has been purified and crystallized and diffraction data have been collected to 2.4 Å resolution. The ChuZ-haemin complex crystals belonged to space group C222(1), with unit-cell parameters a = 106.474, b = 106.698, c = 52.464 Å, α = β = γ = 90°. The asymmetric unit contained one ChuZ monomer, with a Matthews coefficient of 2.58 Å(3) Da(-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Campylobacter jejuni / enzymology*
  • Crystallization
  • Crystallography, X-Ray
  • Heme Oxygenase (Decyclizing) / chemistry*
  • Heme Oxygenase (Decyclizing) / metabolism
  • Protein Binding

Substances

  • Heme Oxygenase (Decyclizing)