Towards a rapid molecular diagnostic for melioidosis: Comparison of DNA extraction methods from clinical specimens

J Microbiol Methods. 2012 Jan;88(1):179-81. doi: 10.1016/j.mimet.2011.10.023. Epub 2011 Nov 12.

Abstract

Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Burkholderia pseudomallei / chemistry
  • Burkholderia pseudomallei / genetics
  • Burkholderia pseudomallei / isolation & purification*
  • DNA, Bacterial / blood
  • DNA, Bacterial / genetics
  • DNA, Bacterial / isolation & purification*
  • Humans
  • Melioidosis / diagnosis*
  • Melioidosis / microbiology
  • Pathology, Molecular / methods*
  • Real-Time Polymerase Chain Reaction

Substances

  • DNA, Bacterial