Rapid detection of blaIMP-6 by amplification refractory mutation system

Shizuo Kayama; Norifumi Shigemoto; Ryuichi Kuwahara; Makoto Onodera; Michiya Yokozaki; Hiroki Ohge; Fuminori Kato; Jyunzo Hisatsune; Motoyuki Sugai

doi:10.1016/j.mimet.2011.10.022

Rapid detection of blaIMP-6 by amplification refractory mutation system

J Microbiol Methods. 2012 Jan;88(1):182-4. doi: 10.1016/j.mimet.2011.10.022. Epub 2011 Nov 15.

Authors

Shizuo Kayama¹, Norifumi Shigemoto, Ryuichi Kuwahara, Makoto Onodera, Michiya Yokozaki, Hiroki Ohge, Fuminori Kato, Jyunzo Hisatsune, Motoyuki Sugai

Affiliation

¹ Project Research Center for Nosocomial Infectious Disease, Hiroshima University, 1-2-3 Kasumi Minami-ku, Hiroshima 734-8551, Japan.

PMID: 22108497
DOI: 10.1016/j.mimet.2011.10.022

Abstract

Klebsiella pneumoniae resistant to almost all ß-lactams except imipenem designated as ISMRK (imipenem-susceptible meropenem-resistant Klebsiella) is emerging in Japan. All ISMRK carries bla(IMP-6) which differs from bla(IMP-1) by only a single nucleotide at position 640. We devised a rapid detection system of bla(IMP-6) by using ARMS PCR.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Anti-Bacterial Agents / pharmacology
Bacterial Proteins / genetics*
Drug Resistance, Bacterial
Humans
Imipenem / pharmacology
Japan
Klebsiella Infections / microbiology*
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / enzymology
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / isolation & purification*
Microbial Sensitivity Tests
Mutation*
Polymerase Chain Reaction / methods*
beta-Lactamases / genetics*

Substances

Anti-Bacterial Agents
Bacterial Proteins
Imipenem
beta-Lactamases