Abstract
Using real-time polymerase chain reaction (RT-PCR), we measured mRNA amounts of matrix metalloproteinases (MMPs): MMP-1, MMP-2, MMP-9, and MMP-12 genes in psoriatic lesions and unaffected skin of the same patients. We observed significant (about 15-fold) increase in the expression level of matrix metalloproteinase MMP-1 and MMP-12 genes associated with psoriasis. The results of our studies of MMP gene expression in cultured primary human keratinocytes treated with interleukin (IL-17) have shown upregulation of MMP gene expression both in cultured keratinocytes and in psoriatic skin lesions. Therefore, upregulation of MMP genes in the skin affected by psoriasis could result from IL-17 effects on skin cells.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Cells, Cultured
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Female
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Gene Expression Regulation, Enzymologic*
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Humans
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Interleukin-17 / metabolism
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Keratinocytes / enzymology
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Keratinocytes / pathology
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Male
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Matrix Metalloproteinase 1 / biosynthesis*
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Matrix Metalloproteinase 12 / biosynthesis*
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Matrix Metalloproteinase 2 / biosynthesis*
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Matrix Metalloproteinase 9 / biosynthesis*
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Middle Aged
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Psoriasis / enzymology*
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Psoriasis / pathology
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Reverse Transcriptase Polymerase Chain Reaction
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Skin / enzymology*
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Skin / pathology
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Up-Regulation*
Substances
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Interleukin-17
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Matrix Metalloproteinase 2
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Matrix Metalloproteinase 9
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Matrix Metalloproteinase 12
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Matrix Metalloproteinase 1