Site-specific conjugation of ScFvs antibodies to nanoparticles by bioorthogonal strain-promoted alkyne-nitrone cycloaddition

Miriam Colombo; Silvia Sommaruga; Serena Mazzucchelli; Laura Polito; Paolo Verderio; Patrizia Galeffi; Fabio Corsi; Paolo Tortora; Davide Prosperi

doi:10.1002/anie.201106775

Site-specific conjugation of ScFvs antibodies to nanoparticles by bioorthogonal strain-promoted alkyne-nitrone cycloaddition

Angew Chem Int Ed Engl. 2012 Jan 9;51(2):496-9. doi: 10.1002/anie.201106775. Epub 2011 Nov 25.

Authors

Miriam Colombo¹, Silvia Sommaruga, Serena Mazzucchelli, Laura Polito, Paolo Verderio, Patrizia Galeffi, Fabio Corsi, Paolo Tortora, Davide Prosperi

Affiliation

¹ NanoBioLab, Dipartimento di Biotecnologie e Bioscienze, Università di Milano Bicocca, Piazza della Scienza 2, 20126 Milano, Italy.

PMID: 22121095
DOI: 10.1002/anie.201106775

Abstract

Particularly suitable: An N-terminal serine mutant of anti-HER2 scFv antibody was conjugated to polymer-coated magnetofluorescent nanoparticles by strain-promoted alkyne-nitrone cycloaddition. The resulting nanoparticles (see scheme) proved effective in targeting and labeling HER2-positive breast cancer cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkynes / chemistry
Cell Line, Tumor
Cyclization
Humans
Mutation
Nanoparticles / chemistry*
Nitrogen Oxides / chemistry*
Receptor, ErbB-2 / analysis*
Receptor, ErbB-2 / immunology*
Single-Chain Antibodies / chemistry*
Single-Chain Antibodies / genetics
Single-Chain Antibodies / immunology*

Substances

Alkynes
Nitrogen Oxides
Single-Chain Antibodies
nitrones
Receptor, ErbB-2